The Binding Site Group
Immunodiagnostic Systems Merck
Olympus Corporation Nova Century
Scientific Thermo Fisher Scientific
DiaSorin Randox Laboratories
Differentiation of mononuclear cells towards osteoclasts, but don’t yield information concerning the bone resorptive action of the osteoclasts formed.
The action of the osteoclasts is quantified in the pit formation assay. With this particular pupose, the co-culture and disease of cells have been done as described for assays described above with the difference that a bone-like substrate is found in the base of the well where the co-culture is done.
The co-culture is done on the bone such as substrate for 14 times. Cells are then eliminated by treatment with sodium hypochlorite and also the area resorbed from the osteoclasts (the resorption pit) could be evaluated microscopically. The treatment of the surface of the dentin slit can facilitates this with blue. In another assay set up, the consequence of this disease of the osteoclast precursor cells (PBMCs or BMMCs) using a TARGET virus onto its being able to distinguish towards an osteoclast is quantified in a monoculture assay. 1 day after seeding, the cells have been contaminated with TARGET Ad-siRNAs. Four days after infection, recombinant RANKL is inserted into the wells (25ng/ml, R&D programs ). Twice per week, Moderate is refreshed. Fourteen days following addition of RANKL, this monocytes towards osteoclasts’ distinction is quantified using one of those readouts clarified for the assay setup. This enables the identification of variables which are crucial for precursor cells’ reaction into RANKL or M-CSF. PBMC isolation PBMCs are derived from peripheral blood.
Calcium carbonate coating, OAAS™, Gentaur; Biocoat and Osteologic
Ortho practical Diagnostics
Immunofluorescence Chemiluminescence ELISA Radioimmunoassay Segmentation par Application: Infectious Diseases Autoimmune Diseases AIDS